The effect of hydro-alcoholic extracts of Nigella sativa, Carum carvi, Taraxacum officinale, and royal jelly on the survival of HDF cell line under H2O2-induced oxidative stress compared to metformin

Background and aims: Highly active oxygen causes DNA or protein damage. This study aimed to determine the effect of extract three medicinal plants, royal jelly, metformin on survival human dermal fibroblast (HDF) cells under oxidative stress induced by H2 O2 . Methods: Hydroalcoholic extracts were prepared from Nigella sativa, Carum carvi seeds, Taraxacum officinale flower. The growth curve HDF was plotted in Dulbecco’s modified eagle medium (DMEM). exposed treated with different concentrations extracts, metformin. viability then calorimetrically analyzed during times 69-well plates. Results: exponential phase observed 24–72-hour growth. High cell survivals seen 72 hours exposure 25 mg/mL jelly (99.13±3.22%), 15 C. N. sativa (97.87±3.25% 96.22±5.47%, respectively), 10 (96.14±4.72%), 16 (91.23±5.34%), 8 T. (86.31±7.44%), respectively. These results significantly higher than 48 24 (P=0.013), (P=0.019), (P=0.027). Moreover, (25 mg/mL) indicated greater compared highest at (P=0.016-0.032). Conclusion: Royal revealed remarkable effects stressed cells; therefore, they can be proposed for treatment wounds burns after clinical analysis.